Cation-binding Capacity of Membranes Isolated from Micrococcus lysodeikticus
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چکیده
منابع مشابه
Immunological properties of Micrococcus lysodeikticus membranes.
Membranes of Micrococcus lysodeikticus possess antigens which are distinct from other cellular components such as cytoplasm, ribosomes, and cell walls. Only a few (two to three) components are found when dissociated membranes are examined by immunodiffusion and immunoelectrophoresis techniques. Membranes treated with 0.3% sodium dodecyl sulfate, 0.3% Triton X-100, trypsin, phospholipase A or C,...
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Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polypeptide possessed a covalently bound flavin prosthetic group and appeared to be ...
متن کاملMembrane Fractions from Micrococcus lysodeikticus
1. A method is described for the isolation of pure mesosomal membrane fractions from Micrococcus lysodeikticus. 2. Plasmolysis of cells, before wall digestion, was necessary for effective mesosome release. 3. The effect of mild shearing forces, temperature and time upon the release of mesosomal membrane from protoplasts was investigated. 4. The optimum yield of mesosomal membranes from stable p...
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Beers, Roland F., Jr. (Johns Hopkins University, Baltimore, Md). Acridine orange binding by Micrococcus lysodeikticus. J. Bacteriol. 88:1249-1256. 1964.-Micrococcus lysodeikticus cells bind acridine orange (AO) reversibly. The adsorption isotherm is consistent with a highly cooperative-type binding similar to that observed with polyadenylic acid. The cells exhibit a strong buffering action on t...
متن کاملBinding of bacitracin to cells and protoplasts of Micrococcus lysodeikticus.
Bacitracin was tritiated by the Wilzbach technique and purified by carboxymethylcellulose chromatography. Binding studies between [3H]bacitracin and Micrococcus lysodeikticus cells and protoplasts indicated that the cells or protoplasts were saturated at 2 X lo5 molecules per cell. This value correlates well with the number of CS5-isoprenyl pyrophosphate molecules found in the membrane and adds...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.100.1.123-127.1969